The malS-5′ UTR regulates hisG, a key gene in the histidine biosynthetic pathway in Salmonella enterica serovar Typhi.

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dc.contributor.author Zhang, Y.
dc.contributor.author Yan, D
dc.contributor.author Xia, L.
dc.contributor.author Zhao, X.
dc.contributor.author Osei-Adjei, G.
dc.contributor.author Xu, S.
dc.contributor.author Huang, X.
dc.date.accessioned 2022-09-08T10:20:33Z
dc.date.available 2022-09-08T10:20:33Z
dc.date.issued 2017
dc.identifier.other 10.1139/cjm-2016-0490
dc.identifier.uri http://atuspace.atu.edu.gh:8080/handle/123456789/218
dc.description.abstract Bacterial noncoding RNAs (ncRNA) regulate diverse cellular processes, including virulence and environmental fitness. The malS 5' untranslated region (named malS-5'UTR) was identified as a regulatory ncRNA that increases the invasive capacity of Salmonella enterica serovar Typhi. An IntaRNA search suggested base pairing between malS-5'UTR and hisG mRNA, a key gene in the histidine biosynthetic pathway. Overexpression of malS-5'UTR markedly reduced bacterial growth in minimal medium without histidine. Overexpression of malS-5'UTR increased mRNA from his operon genes, independently of the bax gene, and decreased HisG protein in Salmonella Typhi. RNA structure analysis showed base pairing of the malS-5'UTR RNA with the hisG mRNA across the ribosome binding site. Thus, we propose that malS-5'UTR inhibited hisG translation, probably by base pairing to the Shine-Dalgarno sequence. en_US
dc.language.iso en en_US
dc.publisher PubMed en_US
dc.relation.ispartofseries vol;63
dc.subject ARNnc en_US
dc.subject Salmonella enterica serovar Typhi en_US
dc.subject Salmonella enterica sérovar Typhi en_US
dc.subject hisG en_US
dc.subject histidine en_US
dc.subject malS-5′UTR en_US
dc.subject ncRNA en_US
dc.title The malS-5′ UTR regulates hisG, a key gene in the histidine biosynthetic pathway in Salmonella enterica serovar Typhi. en_US
dc.type Article en_US


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